Fig. 2 Six SASE genes are commonly required to sustain SNC survival. b–d Survival of IR- b, REP- c, and OI-SNCs d at day 6 afterSASE gene knockdown with two independent shRNAs per gene. Survivalwas measured by MTS assay. Comparisons were made to SNCs of samecultures infected with scrambled shRNA (shScr; 100% line). Scale bar,200 μm. Data represent means ± SEM. n depict independent MEF lines thatwere pooled from 2 to 4 independent experiments. Statistics: one-wayANOVA with Sidak’s correction in b–d.
Fig. 3 SASE genes implicated in SNC survival have limited impact on the viability of cycling cells. a Quantification of TUNEL-positive IR-senescent orproliferating cells 4 or 6 days after shRNA-mediated SASE gene knockdown. We note that due to the experimental setup some shScr control values areused for several comparisons when they were assessed in the same experiment. b Increase in TUNEL-positive cells 4 and 6 days after lentiviral infectionrelative to cells containing scrambled shRNA. Data from a were used for this calculation. Data represent means ± SEM. n = 6 independent MEF lines pooledfrom 2 independent experiments in a, b. Statistics: one-way ANOVA with Sidak’s correction in a, paired, two-tailed t-tests in b.
Fig. 4 Suppression of p53-dependent and -independent pathways contribute to SNC survival. a Cell survival as measured by MTS assay, of IR-, REP- andOI-SNCs at day 6 after knockdown of the indicated genes. Comparisons were with senescent MEFs containing Scr-shRNA or Scr- and p53-shRNA (100%line).
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