这个工具出手就是CNS级水准!师妹师弟都在偷偷用!
分享一个强大的R工具—scop,scop是single-cell omics data分析工具合集,包含scRNA-seq和 scATAC-seq data等分析方法,围绕Seurat对象构建,少量代码完成你的任务!
使用一下scop
Differential expression analysis
pancreas_sub <- RunDEtest(
srt = pancreas_sub,
group_by = "CellType",
fc.threshold = 1,
only.pos = FALSE
)
VolcanoPlot(
srt = pancreas_sub,
group_by = "CellType"
)
Enrichment analysis
CellDimPlot
CellStatPlot
Other visualization
Cell annotation using single-cell datasets
ht <- CellCorHeatmap(
srt_query = pancreas_sub,
srt_ref = panc8_rename,
query_group = "SubCellType",
ref_group = "celltype",
nlabel = 3,
label_by = "row",
show_row_names = TRUE,
show_column_names = TRUE
)
print(ht$plot)
更多。。。。。。:https://github.com/mengxu98/scop
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